采用基于PAS(PCR-based Accurate Synthesis)的方法合成target-gene基因,双酶切连接至pFast-bac1载体的BamH I和Hind III之间;将获得的重组质粒转入TOP10克隆菌株,挑取阳性克隆子测序,测序结果拼接如下所示,单划线区为目的基因基因区域。紫色区域为酶切位点,黄色区域为GP67信号肽,绿色区域为6XHis标签;
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