目的：筛选出产高活力糖化酶的优良菌株；方法：经过紫外线与光复活交替处理单孢子悬液，利用透明圈法和摇瓶发酵进行初筛和复筛；结论：筛选出一株菌株12，通过摇瓶对照实验发现，其酶活达到了5544.3U/mL，比出发菌株提高了25.41%，效果较显著，选育得到的菌株经遗传稳定性试验表明有较好的遗传稳定性。
关键字：紫外诱变；糖化酶；黑曲霉；菌种选育
Aim: To isolate high activity saccharifying enzyme.
Methods: The spore suspension were alternately performed by UV and photoreactivation, and the high-producing strain was primary screened by the mothod of transparent circle on plate. The secondary screening was carried out by the flask culturation.
Conclusion: A strain 12 was obtained by UV mutagenesis. Using flask culturation, the enzyme activity was 5544.3 U/mL, which was 25.41% higher than of control strain, and the effect was significant. The mutanted strain identified by the test of genetic stability showed favorable stability.
Key words: UV mutagenesis, Saccharifying enzyme, Aspergillus niger,
Strain breeding
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参考知识1
Purpose: high energy saccharifying enzyme screening of fine strains, Methods: by ultraviolet light and resurrection single spore suspension liquid alternant processing, use transparent circle and wave screening for early fermentation and screen, Conclusion: the strains isolated by 12, placebo-controlled trial flask, reached the enzyme 5544.3 U/mL, starting 25.41% improved than strains, the effect is remarkable, breeding of the genetic stability test shows that strains have good genetic stability.
Key words: uv mutagenesis, Saccharifying enzyme, Aspergillus, Strain